Used to stain microorganisms from cultures or specimens by the differential gram method. Produces gram stains with brighter more distinct colors. Hardy Diagnostics Gram Stain Kit Advanced (Cat. no. GK400AS) is comprised of Advanced Crystal Violet, Stabilized Povidine, Fast Decolorizer and Advanced Counterstain. Advanced Crystal Violet has been shown to provide superior, consistent and brighter staining of gram positive organisms, especially those which decolorize easily. Advanced Counterstain, a stronger counterstain than Safranin, will stain the gram negative organisms a very deep red. Contrast between gram positive organisms and gram negative organisms in a mixed field is greatly enhanced. The advanced system helps to guard against accidental over decolorization, thus reducing the possibility of mistaking a gram positive organism for a gram negative one. The Gram Stain is one of the most basic of all bacteriologic tests and allows bacteria to be grouped according to their staining reaction, an invaluable aid in bacterial identification. The difference in staining reaction between gram positive and negative bacteria is attributed to the distinctive chemical and physical structure of the cell walls of these organisms. This differential stain is usually described as being related to the higher lipid content of gram negative bacterial cell walls. Some suggest it is instead due to a reaction with a magnesium ribonucleate protein complex. Contents: (1) Hucker's Crystal Violet (60 milliliter) (1) Povidine, stabilized (60 milliliter) (1) Decolorizer, Fast (60 milliliter) (1) Counterstain, Advanced (60 milliliter) (1) Instructions and material safety data sheet (MSDS) packet. The Hardy Diagnostics manufacturing facility and quality management system comply with cGMP's and have achieved ISO 13485 certification as a device manufacturer.